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Other artifacts may conceivably arise from esterification of acidic amino acid residues when extraction is performed with an alcohol-acid solvent system or from deamidation of acid-amide groups. 4. 1. Noncolumn Methods for Free Peptides A sensitive and reproducible procedure for the detection of amino acids and peptides on thin-layer chromatograms has been developed by Felix and Jimenez (1974). 05% in acetone), and then again with triethylamine. By washing paper chromatograms with acetone, both before and after reacting with fluorescamine, Mendez and Lai (19750) have achieved a sensitivity for peptides below 100 pmo!.

72:619-622. , 1966, Accelerate automatic chromatographic analysis of peptides on a spherical resin, Anal. Biochem. 16:91-106. , 1975, Enzymatic inactivation of Substance P by a partially purified enzyme from rat brain, Biochem. Biophys. Res. Commun. 65:153-160. , 1973, Fluorimetric assay of proteins in the nanogram range, Arch. Biochem. Biophys. 155:213-220. , 1975, Automatic monitoring of primary amines in preparative column effluents with fluorescamine, Anal. Biochem. 67:438-445. , 1975, Quantitative analysis of amino acids and peptides in the femtomolar range, Anal.

65:312-316. , 1976, High efficiency cation exchange chromatography of polypeptides and polyamines in the nanomole range, 1. Chromatogr. in press. , 1974, Fluorometric scanning of ftuorescamine-Iabeled proteins in polyacrylamide gels, Anal. Biochem. 59:24-33. , 1972, An accurate method for correcting unknown amino acid losses from protein hydrolyzates, Anal. Biochem. 48:233-246. , 1975, Peptides: An analytical method for their resolution by polyacrylamide gel electrophoresis applicable to a wide range of sizes and solubilities, Anal.

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