Practical Problem Solving in HPLC by Stavros Kromidas PDF

By Stavros Kromidas

ISBN-10: 3527298428

ISBN-13: 9783527298426

Quickly and simple strategies to HPLC difficulties! For forty five ordinary questions of HPLC clients, the reply and normal conclusions are provided on not more than four pages every one. The ebook covers - easy apparatus checks and choice standards for columns, buffers and so on. - particular difficulties and the way to unravel them - techniques for optimizing separations. The publication additionally contains a specified bankruptcy at the retention of ionizable parts in RP-HPLC, references, info tables and payment lists. it is a first-aid equipment for each HPLC consumer. Stavros Kromidas is coping with director of NOVIA, a firm delivering consulting companies and coaching classes for analytical laboratories. for 5 years he used to be revenues supervisor for the HPLC producer Waters in Germany. he's writer and editor of diverse books.

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R. Snyder, Troubleshooting LC Systems, Human Press, 1989). 32 Table 9-1. Volumes of capillary units as a function of their internal diameter (in mm and inches). 72 The left hand column gives possible capillary internal diameters in inches, and the second column gives the conversions to mm. The volumes of the capillaries in Ill/cm are given in the right hand column. 4 Ill. 13 mm capillary you are safe, at least concerning the capillary volumes. Be aware of additional capillaries such as a heat exchanger capillary in your detector, that has a volume that you cannot define.

If the stationary phase and/or the solutes contain cyclic structures, large entropy differences are possible. In this case, the selectivity is strongly dependent on temperature. 2. A steric "relationship" of sample and stationary phase is often more advantageous for selectivity than a chemical relationship. The selectivity is increased if similar structural elements are present in both stationary phase and solutes. Finally, further advice in this context: If you are interested in an analysis of characteristics of your particular compounds or stationary phases, you should consider van't Hoff plot, which are suitable for these questions.

Well, within reason. First rule of thumb: (for the more stringent users among us): The dead volume of a very good isocratic instrument should be about 1/6 of the peak volume. 6 mm, the peak volume is in the order of 100-120 /11. In this case, a dead volume of 20-30/11 is OK. 4 Figure 9-1. Measurement of the dead volume. Second rule of thumb (for the more generous users): The dead volume should not exceed the detection cell volume by more than 5-6 times. Using the most common detection cells of 8-12 ~l, dead volumes should not exceed 50-60 ~1.

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