By Duncan E. McRee, Peter R. David
ISBN-10: 0124860524
ISBN-13: 9780124860520
Designed for simple use via either starting and skilled protein crystallographers, the second one version of useful Protein Crystallography is a necessary instruction manual for any scientist attracted to fixing a protein constitution. The ebook contains examples of exact experiments and knowledge, electron density maps, and desktop tools. This moment version has new fabric protecting CCP4, SHELX, cryocrystallography, MAD and automatic becoming. Key beneficial properties* In-depth assurance of each point of crystallography* insurance of the small information which may make or holiday initiatives* Strongly application-oriented* awfully good illustrated* basic and easy-to-follow strong equipment* Tutorials with genuine information on hand on the net* beneficial for a wide spectrum of scientists
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Additional info for Practical Protein Crystallography, Second Edition
Sample text
The first step to try is a very fine scan of conditions nearest those used initially to find the optimal conditions for growing only a few large crystals in a single setup. Vary the precipitant concentration, the protein concentration, the pH, and the temperature. You may also want to try varying the buffer used and its concentration. Using different types of setup will vary the equilibration rate, which can often lead to improved growth. What are needed are conditions where nucleation is rare and crystal growth is not too rapid.
This is very common for proteins. If they crystallize under one condition, chances are they will crystallize under another condition in a different space group and in a different habit. If there were only one condition out of all possible ones, I doubt that very many proteins would ever crystallize. 3 . . . CRYSTAL STORAGE A N D H A N D L I N G Protein crystals can be stored for a few years and still diffract. Some precautions will help increase lifetime. Some proteins can be simply left in the drops in which they grew.
Wiley, New York. 3 X-RaySources 37 2 g E 3 A t ! i t I FIG. 10 Production of X-rays. Electrons are accelerated in a vacuum to strike a copper target. (1) The electron acceleration voltage is below the threshold for characteristic radiation, and the X-rays produced have wavelengths (A) corresponding to the energy of the electrons: A = h c / e V = 12,398/V. (2) When the electrons exceed a certain voltage, they will have enough energy to displace a K-shell electron. X-rays are then produced as electrons fall from the L-shell (K~) of M-shell (K~), producing sharp peaks of X-rays superimposed on the white radiation.
Practical Protein Crystallography, Second Edition by Duncan E. McRee, Peter R. David
by David
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