X-Ray and Neutron Diffraction in Nonideal Crystals - download pdf or read online

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Sample text

Measurements can be performed by batch methods, a5'16 with microcolumns filled with crystals, 17 or by scintillation. ~8 Most solubility data are given with an accuracy better than 10%. Nevertheless, these solubility determinations required to quantify the effects of a given parameter are tedious and consume both time and protein. They are necessary to clarify the effect of crystallization parameters on the model protein solubility, which can then be transposed for the crystallization of a new protein.

W. , and Y. Yin, Acta Crystal. D50, 572-590 (1994). 28 C. W. , Methods Enzymol. 276, [5], 1997 (this volume). 29 M. J. Cox and P. Weber, J. Appl. Cryst. 20, 366 (1987). 3o A. Ducruix and R. " IRL Press/Oxford University Press, Oxford, 1992. 31 p. C. Weber, Adv. Protein Chem. 41, 1 (1991). 32 j. S. Punzi, J. Luft, and V. Cody, J. Appl. Cryst. 24, 406-408 (1991). 33j. Luft and V. Cody, J. Appl. Cryst. 22, 396 (1989). [3] 23 CRYSTALLOGENESIS AND PRECRYSTALLINE STATE [3] I n f e r e n c e s D r a w n f r o m P h y s i c o c h e m i c a l S t u d i e s Crystallogenesis and PrecrystaUine State of By MADELEINE RIES-KAUTr and ARNAUD DUCRU1X Determination of the three-dimensional structure of a protein by X-ray crystallography first requires the preparation of crystals.

Lafond, S. Vessler, J. P. Astier, and R. Boistelle, J. Cryst. Growth 143, 249 (1994). 23S. Lafond, S. Vessler, J. P. Astier, and R. Boistelle, J. Cryst. Growth, (in press). 24S. B. Howard, P. J. Twigg, J. K. Baird, and E. J. Meehan, Z Cryst. Growth 90, 94 (1988). _p. Guilloteau, M. Ri~s-Kautt, and A. Ducruix, J. Cryst. Growth 122, 223 (1992). 34 CRYSTALS [31 Effect of pH on Protein Solubility Equation (3) predicts a decrease of solubility when approaching the pI because of the decrease of protein net charge, for a given ionic strength.

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