By Donald R. Helinski
ISBN-10: 1461294878
ISBN-13: 9781461294870
ISBN-10: 1461324475
ISBN-13: 9781461324478
The examine of bacterial plasmids has now not constantly been as renowned because it is at the present time. for a few years, the molecular biology of professional cary otes was once targeted seriously on bacteriophage and plasmid investigations which have been conducted in just a number of laboratories. no matter what inter est existed in plasmids involved the function of those extrachromosomal parts in bacterial conjugation, genetic exchanges, and antibiotic resistance, in addition to within the constitution of plasmids themselves. progressively, even if, it grew to become more and more glaring that a number of the detailed features displayed by way of micro organism of scientific, agricul tural, commercial, and environmental value are made up our minds through genes carried through plasmids, and this curiosity in plasmid-encoded capabilities, comparable to bacterial virulence houses (exotoxin produc tion, serum resistance, adhesiveness), metabolism of natural com kilos, plant tumor formation, and organic nitrogen fixation, ended in expanding learn of. the plasmids that hold those genes. Inves tigations of different plasmid-related homes resembling replication and recombination have yielded a lot information regarding primary organic methods; details having implications that stretch a ways past the actual plasmids less than learn. simultaneously, plas mids have been enjoying a key function within the discovery of bacterial transpos capable components and have been proving to be more and more important within the elu cidation of mechanisms liable for various chromosomal rearrangement occasions in micro organism and crops. Their prestige as "mini chromosomes" that may be remoted simply from bacterial cells after which reintroduced into different cells through transformation is of fundamen tal value during this regard.
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Example text
As with IS50. the simple insertions appear to arise by events in which the element failed to duplicate. Thus. recent results with Mu and with lSI call for the consideration of new models. ones in which transpositIon is sometimes conservative and sometimes replicative. 41 MECHANISMS OF TRANSPOSITION We propose that a single intermediate can lead to cointegrate or noncointegrate transposition products as depicted in Fig. 6. In this model the element and target DNAs are joined by single strands, generating incipient replication forks which are susceptible to nucleolytic attack.
64. DATTA replicon of IncFI plasmids that has homology with a basic replicon of IncFII plasmids. Plasmid. J. B. Formal (1982) Involvement of a plasmid in the invasive ability of Schigella flexneri. Infect. Immun. 35:852-860. Schottel. J. N. Cohen (1981) Cloning and expression in Streptomyces lividans of antibiotic resistance genes derived from Escherichia coli. J. Bact. 146:360-368. , and I. Phillips (1982) Mechanisms of resistance to aminoglycosides in clinical isolates. J. Antimicrob. Chemother.
With lSI. as with IS50. the simple insertions appear to arise by events in which the element failed to duplicate. Thus. recent results with Mu and with lSI call for the consideration of new models. ones in which transpositIon is sometimes conservative and sometimes replicative. 41 MECHANISMS OF TRANSPOSITION We propose that a single intermediate can lead to cointegrate or noncointegrate transposition products as depicted in Fig. 6. In this model the element and target DNAs are joined by single strands, generating incipient replication forks which are susceptible to nucleolytic attack.
Plasmids in Bacteria by Donald R. Helinski
by Charles
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